Publication list of Ralf Pörtner

 

  • Cantürk, C.; Baade U, Salazar R, Storm N, R. Pörtner; W. Hoeppner. Genotyping of CYP21A1P in German Population as an Aid to Molecular Biological Analysis of Congenital Adrenal Hyperplasia. Clinical chemistry. DOI: 10.1373/clinchem.2010.156893


  • Lutz, V; M. Sattler; St. Gallinat; H. Wenck; R. Pörtner; F. Fischer. GImpact of collagen crosslinking on the second harmonic generation signal and the fluorescence lifetime of collagen autofluorescence. Skin Research and Technology (2011), DOI: 10.1111/j.1600-0846.2011.00549.


  • Sercinoglu, O., Platas Barradas, O., Sandig, V., Zeng, A.P., Pörtner, R. DoE of fed-batch processes – model-based design and experimental evaluation. BMC Proceedings 2011, 5(Suppl 8):P46


  • Platas Barradas, O., Jandt, U., Phan, L.D.M., Villanueva, M., Rath, A., Reichl, U., Schräder, E., Scholz, S., Noll, T., Sandig, V., Pörtner, R., Zeng, A.P. Criteria for bioreactor comparison and operation standardisation during process development for mammalian cell culture. BMC Proceedings 2011, 5(Suppl 8):P47


  • Schaletzky, M., Platas Barradas, O., Sievert, H., Balabanov, S., Zeng, A.P., Pörtner, R. Cultivation strategies of a BA/F3 cell line for fundamental cell research. BMC Proceedings 2011, 5(Suppl 8):P48


  • Platas Barradas, O., Jandt, U., Hass, R., Kasper, C., Sandig, V., Pörtner, R., Zeng, A.P. Physical methods for synchronization of a human production cell line. BMC Proceedings 2011, 5(Suppl 8):P49


  • Goepfert, C., Scheurer, W., Rohn, S., Rathjen, B., Meyer, S., Dittmann, A., Wiegandt, K., Janßen, R., Pörtner, R. 3D-Bioreactor culture of human hepatoma cell line HepG2 as a promising tool for in vitro substance testing. BMC Proceedings 2011, 5(Suppl 8):P61


  • Pörtner, R., Platas-Barradas, O., Gradkowski, J., Gautam, R., Kuhnen, F., Hass, V. “BioProzessTrainer” as training tool for design of experiments. BMC Proceedings 2011, 5(Suppl 8):P62


  • Platas Barradas, O., Pörtner, R., Jandt, U., Phan, L.D.M., Villanueva, M.E., Rath, A., Reichl, U., Schräder, E., Scholz, S. , Noll, T., Sandig, V., Zeng, A.-P. (2011). Evaluation of criteria for bioreactor comparison and operation standardisation for mammalian cell culture. (submitted)


  • Weber, Ch.; D. Freimark; R. Pörtner; P. Pino-Grace; S. Pohl; Ch. Wallrapp; P. Geigle; P. Czermak: Expansion of human mesenchymal stem cells in a fixed-bed bioreactor system based on non-porous glass carrier – Part A: Inoculation, cultivation, and cell harvest procedures. Int J Artif Organs 33 (8): 512-525 (2010)


  • Weber, Ch.; D. Freimark; R. Pörtner; P. Pino-Grace; S. Pohl; Ch. Wallrapp; P. Geigle; P. Czermak: Expansion of human mesenchymal stem cells in a fixed-bed bioreactor system based on non-porous glass carrier – Part B: Modeling and scale up of the system". Int J Artif Organs 33 (11): 782 – 795 (2010


  • Hissnauer, T.N.; A. Baranowsky; J.M. Pestka; T. Streichert; K. Wiegandt; C. Goepfert; F.T. Beil; J. Albers; J. Schulze; P. Ueblacker; J.P. Petersen; T. Schinke; N.M. Meenen; R. Pörtner; M. Amling: Identification of Molecular Markers for Articular Cartilage. Osteoarthritis and Cartilage, 18 (12): 1630 -1638 (2010)


  • Pörtner, R.; Ch. Burger; K. Maier-Reif; Ch. Goepfert; S. Hoffmann; A. Scriba und U. Marx: Oranotypic Tissue Cultures for Substance Testing – A Survey of Academic and Industrial Activities in Germany. BIOforum Europe 3: 21-23 (2010)


  • Kasper, C.; M. van Griensven, R. Pörtner (eds.): Strategies for stem cell expansion and differentiation; Series: Advances in Biochemical Engineering / Biotechnology, Vol. 123 (2010), ISBN: 978-3-642-16050-9


  • Goepfert C, Slobodianski A, Schilling AF, Adamietz P, Pörtner R.: Cartilage Engineering from Mesen-chymal Stem Cells. In: Advances in biochemical engineering/biotechnology. , Vol. 123, 163-200 (2010)


  • Freimark, D.; P. Pino-Grace; S. Pohl; C. Weber; C. Wallrapp; P. Geigle; R. Pörtner; P. Czermak: Use of Encapsulated Stem Cells to Overcome the Bottleneck of Cell Availability for Cell Therapy Approaches. Transfus Med Hemother 37:66–73 (2010)


  • Goepfert, Ch.; V. Lutz; S. Lünse; S. Kittel: K. Wiegandt; M. Kammal; K. Püschel; R. Pörtner; Evaluation of cartilage specific matrix synthesis of human articular chondrocytes after extended propagation on microcarriers by image analysis. Int J Artif Organs 33 (4): 204-218 (2010)


  • Pörtner, R.; N.M. Meenen: Technische Aspekte in regenerativer Medizin und Tissue Engineering von Knorpelersatz. HaMiPla Jahrgang 42, Ausgabe 6, Dezember 2010


  • Weber, C.; Pohl, S.; Poertner, R.; Pino-Grace, Pablo; Freimark, D.; Wallrapp, C.; P. Geigle; P. Czermak: Production Process for Stem Cell Based Therapeutic Implants: Expansion of the Production Cell Line and Cultivation of Encapsulated Cells. In: Advances in biochemical en-gineering/biotechnology, Vol. 123, 143-162 (2010)


  • Hass, V. und R. Pörtner: Praxis der Bioprozesstechnik. Spektrum Akademischer Verlag (2009) ISBN: 978-3-8274-1795-4


  • Kasper, C.; M. van Griensven, R. Pörtner (eds.): Bioreactor Systems for Tissue Engineering; Series: Advances in Biochemical Engineering / Biotechnology , Vol. 112 (2009) ISBN: 978-3-540-69356-7


  • Nehring, D; R. Pörtner; M. Schweizer; K. Cichutek; P. Czermak: Integrated inline filtration: A method to produce highly concentrated retroviral vector titer supernatant. Desalination 246: 241-247 (2009)


  • Pörtner, R.; C. Goepfert; K. Wiegandt; R. Janssen; E. Ilinich; H. Paetzhold; E. Eisenbarth; M. Morlock: Technical Strategies to Improve Tissue Engineering of Cartilage Carrier Constructs - A Case Study. Advances in Biochemical Engineering / Biotechnology , Vol. 112: 145-182 (2009)


  • Wiegandt, K; C. Goepfert; T. Richter; D. Fritsch; R. Janßen; R. Pörtner: In vitro generation of cartilage-carrier-constructs on hydroxylapatite ceramics with different surface structures. The open biomedical engineering journal, 2, 64-70 (2008)


  • Hussein, M.A.; S. Esterl; R. Pörtner; K. Wiegandt and T. Becker: On the Lattice Boltzmann Method Simulation of a two Phase Flow Bioreactor for Artificially Grown Cartilage Cells. Journal of Biomechanics 41, 16: 3455-3461 (2008)


  • Eibl, R.; D. Eibl; R. Pörtner; G. Catapano and P. Czermak: Cell and Tissue Reaction Engineering, Springer (2008). ISBN 978-3-540-68175-5


  • Petersen, J.-P.; P. Uebelacker; Ch. Goepfert; P. Adamietz; A. Stork; J.M. Rueger; R. Pörtner; M. Amling and N.M. Meenen: Long term results after implantation of tissue engineered cartilage for the treatment of osteochondral lesions in a minipig model. Journal of Materials Science: Materials in Medicine 9(5):2029-38 (2008)


  • Hussein, M.A.; S. Esterl; R. Pörtner; K. Wiegandt and T. Becker: Impulse and mass transport in a cartilage bioreactor using the lattice Boltzmann method. International Journal of Computational Fluid Dynamics, 22, 341 - 350 (2008)


  • Wiegandt, K. Ch. Goepfert, R. Pörtner: Improving in vitro Generated Cartilage-Carrier-Constructs by Optimizing Growth Factor Combination. The Open Biomedical Engineering Journal, 1, 85-90 (2007)


  • Weber, Ch.; S. Pohl; R. Pörtner; Ch. Wallrapp; M. Kassem; P. Geigle and P. Czermak: Cultivation and Differentiation of Encapsulated hMSC-TERT in a Disposable Small-Scale Syringe-Like Fixed Bed Reactor. The Open Biomedical Engineering Journal, 1: 64-70 (2007)


  • Weber, Ch.; S. Pohl; R. Pörtner; Ch. Wallrapp; M. Kassem; P. Geigle and P. Czermak: Expansion and Harvesting of hMSC-TERT. The Open Biomedical Engineering Journal, 1: 38-46 (2007)


  • R. Pörtner, O.B. Platas, D. Fassnacht, D. Nehring, P. Czermak, H. Märkl: Fixed bed reactors for the cultivation of mammalian cells: design, performance and scale-up. Open Biotechnology Journal, 1: 41-46 (2007)


  • Pörtner, R. (ed.): Animal Cell Biotechnology - Methods and Protocols. Humana Press (2007). ISBN13: 978-1-58829-660-3 ISBN10: 1-58829-660-1


  • Becker, Th; B. Hitzmann; R. Pörtner; K.F. Reardon; F. Stahl and R. Ulber: Future Aspects of Bioprocess Monitoring. in: D. Sell, R. Ulber (Eds.); White Biotechnology; Advances in Biochemical Engineering/Biotechnology; Springer Verlag 105 (2007) 249-293


  • Heyland, J.; K. Wiegandt; Ch. Goepfert; St. Nagel-Heyer; E. Ilinich; U. Schumacher and R. Pörtner: Redifferentiation of chondrocytes and cartilage formation under intermittent hydrostatic pressure. Biotechnology Letters, 28: 1641-1648 (2006)


  • Nehring, D.; R. Gonzales; R. Pörtner and P. Czermak: Experimental and modelling study of different process modes for retroviral production in a fixed bed reactor. Journal of Biotechnology, 122 (2), 239-253 (2006)


  • Nagel-Heyer, St.; Ch. Goepfert; P. Adamietz; N. M. Meenen and R. Pörtner: Cultivation of three-dimensional cartilage-carrier-constructs under reduced oxygen tension. Journal of Biotechnology, 121 (4), 486-497 (2006)


  • Pörtner, R.; Hass, V.C.: Interactive virtual learning environment for biotechnology (eLearnBioTec). Chemie-Ingenieur-Technik, 77(8), 1256 (2005)


  • Hass, V.C.; Kuhnen, F.; Schoop, K.-M.; Poertner, R.: Integration of computer based learning and training in bioprocess technical training. Chemie-Ingenieur-Technik, 77(8), 1250-1251 (2005)


  • Pörtner, R.; St. Nagel-Heyer; Ch. Goepfert; P. Adamietz and N. M. Meenen: Bioreactor design for tissue engineering. Journal of Bioscience and Bioengineering, 100(3): 235-245 (2005).


  • Meenen, N.M., P. Ueblacker, R. Pörtner, C. Göpfert, S. Nagel-Heyer, J. P. Petersen und P. Adamietz: ; "Knorpel aus dem Labor-eine Sackgasse?", Arthroskopie 18: 245-252 (2005)


  • Nagel-Heyer, S.; Ch. Goepfert; P. Adamietz; N.M. Meenen; J.-P. Petersen and R. Pörtner: Flow-chamber bioreactor culture for generation of three-dimensional cartilage-carrier-constructs. Bioprocess and Biosystems Engineering, 27: 273-280 (2005)


  • Frahm, B.; P. Lane, A. Munack und R. Pörtner: Optimierung und Steuerung von Zellkultur-Fed-Batch-Prozessen mittels einer Kollokationsmethode. Chemie-Ingenieur-Technik, 77 (4), 429 - 435 (2005)


  • Nagel-Heyer, S.; Ch. Goepfert; M.M. Morlock and R. Pörtner: Relationship between gross morphological and biochemical data of tissue engineered cartilage-carrier-constructs. Biotechnology Letters 27(3): 187-192 (2005)


  • Frahm, B.; P. Lane, A. Munack und R. Pörtner: Optimierung und Steuerung von Zellkultur-Fed-Batch-Prozessen mittels einer Kollokationsmethode. Chemie-Ingenieur-Technik, 77 (4), 429 – 435 (2005)


  • Pörtner, R.; J.-O. Schwabe and B. Frahm: Evaluation of selected control strategies for fed-batch cultures of a hybridoma cell line, Biotechnology and Applied Biochemistry 40: 47-55 (2004)


  • Pörtner, R.; M. Seemuk; R.-Ch. Schlothauer; D. Elsser: Anaerobe Kultivierung von Lactococcus lactis im Festbettreaktor. Chemie-Ingenieur-Technik, 76, 10: 1599-1602 (2004)


  • Frahm, B., Lane, P.; Märkl, H., Pörtner, R. (2003): Improvement of a mammalian cell culture process by adaptive, model-based dialysis fed-batch cultivation and suppression of apoptosis, Bioprocess and Biosystems Engineering 26, 1-10


  • Frahm, B., Hass, V. C., Lane, P., Munack, A.; Märkl, H.; Pörtner, R. (2003): Fed-batch Kultivierung tierischer Zellen - eine Herausforderung zur adaptiven, modellbasierten Steuerung, Chemie Ingenieur Technik 4, 457 460


  • Frahm, B.; H.-C. Blank; P. Cornand; W. Oelßner; U. Guth; P. Lane; A. Munack; K. Johannsen and R. Pörtner (2002): Determination of dissolved CO2 concentration and CO2 production rate of mammalian cell suspension culture based on off-gas measurement, Journal of Biotechnology 99/2: 133-148


  • Frahm, B.; P. Lane; H. Atzert; A. Munack; M. Hoffmann; V. Hass and R. Pörtner (2002): Automated, adaptive, model-based control by the OLFO-controller for the effective fed-batch cultivation of hybridoma cells, Biotechnology Progress 18: 1095-1103


  • Frahm, B. und R. Pörtner (2002): Reaktor-Abgasmessungen bei Zellkulturen, BIOforum 4,


  • Nagel-Heyer, St. and R. Pörtner (2002): Bioreactor cultivation for three-dimensional cartilage implants. G.I.T. Laboratory Journal 2: 66-67



  • Fassnacht, D.; I. Reimann, R. Pörtner und H. Märkl (2001): Scale-up von Festbettreaktoren zur Kultivierung tierischer Zellen. Chemie Ingenieur Technik 73: 1075 - 1079


  • Pörtner, R.(2000): Achema-Bericht "Zellkulturtechnik", Chemie Ingenieur Technik 72: 1446/7


  • Fussenegger, M., Fassnacht, D., Schwartz, R., Zanghi, J.A., Graf, M., Bailey, J.E., Pörtner, R. (2000): Regulated Overexpression of the Survival Factor bcl-2 in CHO Cells Increases Viable Cell Density in Batch Culture and Decreases DNA Release in Extended Fixed-bed Cultivation, Cytotechnology 32:45-61


  • Fassnacht, D. and R. Pörtner (1999): Experimental and theoretical considerations on oxygen supply for animal cell growth in fixed bed reactors. Journal of Biotechnology 72 (3): 169 - 184


  • Pörtner, R., Fassnacht, D., Märkl, H. (1999): Immobilization of Mammalian Cells in Fixed Bed Reactors, Bioforum Int. 3:140-141


  • Pörtner, R.; D. Fassnacht und H. Märkl (1999):Immobilisierung tierischer und menschli-cher Zellen in Festbettreaktoren. BIOforum 22: 516-517


  • Nehring, D., P. Adamietz, N. M. Meenen and R. Pörtner (1999): Perfusion cultures and modelling of oxygen uptake with three-dimensional chondrocyte pellets. Bio-technology Techniques 13: 701-706


  • Manusadzianas, L.; R. Vitkas; R. Pörtner and H. Märkl (1999): Phytotoxicities of se-lected chemicals and industrial effluents to Nitellopsis obtusa cells, assessed by using a rapid electrophysiological Charophyte test. ATLA, 27: 379-386


  • Schwabe, J.O., Pörtner, R., Märkl, H. (1999), Improving an On-line Feeding Strategy for Fed-batch Cultures of Hybridoma Cells by Dialysis and "Nutrient-split"-feeding, Bioproc. Eng. 20, 475-484.


  • Fassnacht, D., Rössing, S., Singh, R., Al-Rubeai, M., Pörtner, R. (1999), Influence of BCL-2 Expression on Antibody Productivity in High Cell Density Hybridoma Culture Systems, Cytotechnology 30, 95-105.


  • Pörtner, R., Märkl, H. (1998), Dialysis cultures, Appl. Microbiol. Biotechnol. 50, 403-414.


  • Pörtner, R., Lüdemann, I., Reher, K., Neumaier, M., Märkl, H. (1998), Fixed-bed Dialysis Culture of a Transfectoma Cell Line Producing Chimeric Fab-fragments with "Nutrient-split"-feeding Strategy, Biotechnol. Tech. 12, No. 7, 501-505.


  • Schwabe, J.O., Pörtner, R., Märkl, H. (1998), Strategien zur Steigerung der Antikörperproduktion von Hybridomzellen in Fed-Batch und Dialyse-Prozessen, Kurzfassungen 16. DECHEMA-Jahrestagung der Biotechnologen, 68.


  • Fassnacht, D., Rössing, S., Stange, J., Pörtner, R. (1998), Long-term Cultivation of Immortalised Mouse Hepatocytes in a High Cell Density Fixed Bed Reactor, Biotechnology Techniques 12, 25-30.


  • Fassnacht, D., Rössing, S., Franék, F., Al-Rubeai, M., Pörtner, R. (1998), Effect of BCL-2 Expression on Hybridoma Cell Growth in Serum-supplemented, Protein-free and Diluted Media, Cytotechnology 26,No. 3, 219-225.


  • Pörtner, R. (1998), Fixed Bed Reactors for Cultivation of Mammalian cells, American Clinical Laboratory 5, 6-7.


  • Pörtner, R., Lüdemann, I., Reher, K., Neumaier, M., Märkl, H., (1998), Festbett-Dialyse-Kulturen einer Transfektoma-Zellinie zur Produktion eines chimären Antikörperfragmentes mit "Nutrient-Split"-Fütterung, Kurzfassungen 16. DECHEMA-Jahrestagung der Biotechnologen, 67.


  • Pörtner, R., Lüdemann, I., Reher, K., Neumaier, M., Märkl, H. (1997), Dialysis-fixed Bed Cultures for Highly Efficient Production of Chimeric FAb Fragments With Transfectoma Cells in Protein-free Medium, 10th JAACT-Meeting, 5.-8.11.1997, Nagoya, Japan.


  • Pörtner, R. and M. Koop (1997): A model for oxygen supply in fixed bed reactors with immobilized hybridoma cells. Bioprocess Engineering 17: 269-275


  • Pörtner, R.; S. Rössing und D. Fassnacht (1997): Festbettreaktoren mit immobilisierten tierischen und menschlichen Zellen. Bioscope 9: 25-33


  • Pörtner, R.(1997): Festbett-Dialysekulturen mit Hybridom-Zellen zur effizienten Anti-körperproduktion. Bio World 4/97: 21-23


  • Lüdemann, I., Pörtner, R., Neumann, R., Märkl, H., (1997), "Nutrient-split" Feeding Strategy for DialysisCultures of Immobilized Hybridoma and Transfectoma Cells, M.J.T. Carrondo et al. (eds.): Animal Cell Technology: From Vaccines to Genetic Medicine. Kluwer Academic Publishers, The Netherlands, 361-366.


  • Zumbusch, P.v., Pörtner, R., Brun, B., Brunner G., Märkl, H. (1997), On the Use of Electric Fields to Prevent Membrane Fouling of Porocess Integrated Cross Flow Filtration at Hybridoma Cell Cultures, M.J.T. Carrondo et al. (eds.): Animal Cell Technology: From Vaccines to Genetic Medicine. Kluwer Academic Publishers, The Netherlands, 215-220.


  • Fassnacht, D., Rössing, S., Ghaussy, N., Pörtner, R. (1997), Influence of Non-essential Amino Acids on Apoptotic and Necrotic Death of Mouse Hybridoma Cells in Batch Cultures, Biotechnology Letters 19, 35-38.


  • Pörtner, R., Rössing, S., Koop, M., Lüdemann, I., (1997), Kinetic Studies on Hybridoma Cells Immobilized in Fixed Bed Reactos, Biotechnology and Bioengineering 55, No. 3, 535-541.


  • Pörtner, R. (1997), Festbett-Dialysekulturen mit Hybridom-Zellen zur effizienten Antikörperproduktion, Bio World 4, 21-23.


  • Pörtner, R. (1997), Ein Festbett für tierische Zellen, BioTec 4, 22.


  • Pörtner, R., Lüdemann, I., Märkl, H. (1997), Dialysis Cultures with Immobilized Hybridoma Cells for Effective Production of Monoclonal Antibodies, Cytotechnology 23, 39-45.


  • Pörtner, R., Lüdemann, I., Märkl, H. (1996), Dialysekulturen mit immobilisierten tierischen Zellen, Biospektrum 6, 47-49.


  • Pörtner, R., Schäfer, T. (1996), Modelling Hybridoma Cell Growth and Metabolism - A Comparision of Selected Models and Data, Journal of Biotechnology 49, 119-135.


  • Lüdemann, I., Pörtner, R., Schaefer, C., Schick, K., Srámková, K., Reher, K., Neumaier, M., Franék, F., Märkl, H. (1996), Improvement of the Culture Stability of On-anchorage-dependent Animal Cells Grown in Serum-free Media Through Immobilization, Cytotechnology 19, 111-124.


  • Pörtner, R., Schilling, A., Lüdemann, I., Märkl, H. (1996), High Density Fed-batch Cultures for Hybridoma Cells Performed with the Aid of a Kinetic Model, Bioprocess Engineering 15, 117-124.

  • Pörtner, R., Märkl, H. (1995), Festbettreaktoren für die Kultur tierischer Zellen, BIOforum 18, 449-452.


  • Bohmann, A., Pörtner, R., Märkl, H. (1995), Performance of a Membrane Dialysis Bioreactor with Radial-flow Fixed Bed for the Cultivation of a Hybridoma Cell Line, Appl. Microbiol. Biotechnol. 43, 772-780.


  • Ong, C.P., Pörtner, R., Märkl, H., Yamazaki, Y., Yasuda, K., Matsumura, M. (1994), High Density Cultivation of Hybridoma in Charged Porous Carriers, Journal of Biotechnology 34, 259-268.


  • Pörtner, R., Bohmann, A., Lüdemann, I., Märkl, H. (1994), Estimation of Specific Glucose Uptake Rates in Cultures of Hybridoma Cells, Journal of Biotechnology 34, 237-246.


  • Lüdemann, I., Pörtner, R., Märkl, H. (1994), Influence of the pH on the Ammonia Sensitivity of a Murine Hybridoma Cell Line, Animal Cell Technology: Products of today, prospects of tomorrow, 152-154.


  • Pörtner, R., Bohmann, A., Lüdemann, I., Märkl, H. (1994), Metabolic Parameters of a Hybridoma Cell Line in Batch and Continuous Cultivation, Animal Cell Technology: Products of today, prospects of tomorrow, 457-459.


  • Lüdemann, I., Pörtner, R., Märkl, H. (1994), Effect of NH3 on the Cell Growth of a Hybridoma Cell Line, Cytotechnology 14, 11-20.
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